Category Archives: VDAC

´╗┐However, treatment with RepSox or DM induced either no Ca2+ oscillation or only Ca2+ oscillation with very small peak amplitudes in 3D and 2D cultures at day 7 and 14. Open in a separate window Figure 6 Effect of BMP signaling inhibition and ALK5 inhibitor on CPC differentiation in 3D vs 2D culture. with increased number of cardiac Troponin T (cTnT)-positive cells and synchronized intracellular Ca2+ oscillation. In addition, we studied if 3D nanofiber culture can be used as an in vitro model for compound screening by testing a number of other differentiation factors including a ALK5 inhibitor and inhibitors of BMP signaling. This work highlights the importance of using a more relevant in vitro model and measuring not only the expression of marker proteins but also the functional readout in a screen in order to identify the best compounds and to investigate the resulting biology. Many examples across different…

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