Category Archives: Vascular Endothelial Growth Factor Receptors

[PubMed] [Google Scholar]Beckles DM, Smith AM, ap Rees T. encode the same protein. By comparison of the rice EST sequences, two classes could be identified, one of which is homologous to the RGP1 clone described by Dhugga et al. (1997). Each of the isolated wheat cDNA clones is homologous to the other class, designated RGP2. To obtain cDNA clones corresponding to RGP1, a new specific antisense primer was designed. Using this primer, an clones. The complete sequence of the longest clone from each class was determined. To obtain full-length rice cDNAs from both classes, rice cDNA libraries from etiolated shoot and 7-d-old somatic embryo were screened with two EST clones corresponding to both classes. From both classes, one cDNA clone containing the complete coding region was sequenced. The deduced amino acid sequences of both cDNA clones from wheat and rice are shown in Figure ?Figure11 in comparison with the amino…

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Pharmacother. and more difficult strategy of creating gene knockouts. Second of all, microRNA targeting represents a novel and still undeveloped approach toward potential therapeutic applications. Oligonucleotide (ON) analogs inhibit microRNA function essentially by a steric block, RNase H-independent and RISC-independent, antisense mechanism through complementary binding of the ON to the microRNA sequence. The cellular outcome of such binding is still unclear, with reports arguing either in favor of a mechanism based on simple sequestration by stoichiometric complex formation between the mature microRNA and the ON inhibitor (Chan et al. 2005), or in favor of a yet unknown mechanism by which complex formation leads to degradation of the target microRNA (Krutzfeldt et al. 2005, 2007; Esau et al. 2006). A number of ON analog PLX-4720 types have been proposed that provide both metabolic stability as well as good RNA binding, two fundamental requirements for microRNA inhibition. Early literature reports showed that…

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