Open in another window Figure 6 Retinal function evaluated with electroretinography (ERG)
Open in another window Figure 6 Retinal function evaluated with electroretinography (ERG). administrated in regular or CoCl2-induced cultured condition. Remember that halofuginone inhibited CoCl2-induced HIF actions more powerful than two known HIF inhibitors. Mistake bars indicate the typical deviation. HF; halofuginone, Topo; topotecan, DXR; doxorubicin, CoCl2; cobalt chloride. *** < 0.001, Learners = 0.02) suppressed in halofuginone-treated mice in comparison to handles (Body 3A,B). The upregulated retinal hif-1 and the mark genes aside from vegfa-a in post-I/R retinas had been considerably suppressed in treated mice in comparison to handles (hif-1: = 0.028, vegf-a: = 0.084, glut1: = 0.019, pdk1: = 0.026, respectively) (Figure 3C). These outcomes recommended that administration of halofuginone inhibited elevated HIF-1 and upregulated focus on gene appearance in post I/R retinas. Open up in another window Body 3 Suppression of elevated HIF-1 and upregulated focus on genes by halofuginone administration in I/R retinas. (A) Traditional western blotting…