Category Archives: USP

´╗┐Untreated cells were used like a control for basal Neu2 expression. surface and DENV2 NS1 binding to HPMEC monolayers in the presence of different concentrations of DENV2 NS1 (1.25, 2.5, 5, and 10 g/ml) in Fig 2C. Results represent imply fluorescence intensity (MFI) ideals from three self-employed experiments. Grey bars represent relative sialic acid manifestation, determined by normalizing each condition to untreated control cells (relative sialic acid manifestation = sialic acid manifestation in DENV2 NS1-treated monolayers/sialic acid manifestation in untreated control monolayers). The blue collection represents DENV2 NS1 binding.(TIF) ppat.1005738.s001.tif (1.5M) GUID:?16B181A1-9EEE-4EC7-8544-A34D8AD430F6 S2 Fig: Related to Fig 1: DENV2 NS1-induced endothelial hyperpermeability is not a result of LPS contamination and occurs in additional endothelial cell types. (A) TEER assay to evaluate the effect of DENV2 NS1 (5 g/ml) on HMEC-1 endothelial permeability. Relative TEER values from one self-employed experiment performed in duplicate are plotted in the indicated time points. Error…

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