Positive samples in the 2ME-RSAT were shipped to Cornell University for confirmation by Agarose Gel Immunodiffusion (AGID) testing. accepted by AHS during the study period, 943 (8.9%) were selected for testing. Most study dogs arrived from Oklahoma (28%), Alabama (18%), and Minnesota (12%). The median age of study dogs was 1.5 years; 303 (32%) were intact males and 294 (31%) were intact females. Most study dogs were strays (n=716, 76%). Of the total, 22 (3.1%) stray and eight (3.5%) owner-surrendered dogs were presumptively positive by RSAT; one (0.11%) of the stray dogs was positive by 2ME-RSAT and confirmed by AGID. The positive dog was a healthy-appearing 1 year-old neutered male beagle from Texas. Conclusions: The seroprevalence of canine brucellosis in dogs entering Minnesota for adoption from multiple states was low. Never-the-less, GW791343 HCl care must to be taken to consider all potential risks and outcomes of interstate and international dog trade, including the spread of infectious diseases such as canine GW791343 HCl brucellosis. can also infect humans and cause non-specific illnesses, but may also cause serious complications including endocarditis, similar to other species infections. Infection in people assisting with whelping of an infected dog or disposing of aborted or stillborn fetuses has been characterized (Blankenship and Sanford, 1975; Lucero et al., 2010; Nomura et al., 2010; Rifkin et al., 1978; Rumley and Chapman, 1986; Tosi and Nelson, 1982). There are also case reports of infection in people with limited or general dog contact and even some cases who reported no contact with dogs (Dentinger et al., 2015; Lawaczeck et al., 2011; Lucero et al., 2005; Lucero Ephb2 et al., 2010; Marzetti et al., 2013; Munford et al., 1975; Piampiano et al., 2000; Polt et al., 1982; Rousseau, 1985; Swenson et al., 1972; Ying et al., 1999). In these situations, where people have household contact with dogs in the community (i.e., not associated with breeding stock), transmission and infection risk are poorly understood. Canine brucellosis is reportable in Minnesota to the Minnesota Board of Animal Health (BAH), and surveillance responsibilities are shared between BAH and the Minnesota Department of Health (MDH). Between 2009 and 2017, 117 dogs tested positive for canine brucellosis and were determined to be infected with by the BAH. Of 27 dogs for which clinical information was available, 17 (63%) had at least one clinical sign associated with infection; those without clinical signs were tested because of an epidemiologic link to other identified positive dogs or were incidentally identified by routine wellness testing. Of the dogs with clinical signs, a majority (94%) had back pain or lameness with diagnosed or presumed discospondylitis. Most dog owners (80%) elected humane euthanasia of the infected dog. Prior to 2015, all cases of canine brucellosis in dogs reported in Minnesota were related to purposeful dog breeding. In contrast, of the 34 dogs identified with canine brucellosis in Minnesota in 2015 and 2016, 13 were used in purposeful breeding and 21 were rescue dogs not used for breeding. These 21 dogs originated from specific geographic areas in South Dakota (Minnesota Department of Health: unpublished data). Based on the increased number of canine brucellosis reports in rescue dogs, we wanted to explore if canine brucellosis in canine rescue and shelter populations was an emerging public health GW791343 HCl issue. Because canine brucellosis is considered an incurable and lifelong infection in dogs, and animals can potentially remain infectious for years, the presence of antibodies is considered indicative of infection and potential infectivity (Bramlage et al., 2015; National Association of State Public Health Veterinarians, 2012). Seroprevalence studies for antibodies have.
Positive samples in the 2ME-RSAT were shipped to Cornell University for confirmation by Agarose Gel Immunodiffusion (AGID) testing